High-density culture of mouse-human hybridoma cells using a new perfusion culture vessel.
نویسندگان
چکیده
منابع مشابه
Establishment, Culture and Freezing of Human and Mouse Embryonic Stem Cells: a Protocol Guide
Studies of the biology of human embryonic stem cells (hES cells) have developed rapidly over the past nine years since the first reports of their derivation. They clearly offer enormous potential, not only for regenerative medicine, but also for drug discovery and toxicology, human developmental biology and cancer research. Realizing these potentials a better understanding of the fundamental as...
متن کاملThree-Dimensional Culture of Hybridoma Cells Secreting Anti-Human Chorionic Gonadotropin by a New Rolling Culture System
Cell growth rate and production of monoclonal antibody (MAb) of hybridoma cells producing anti-human chorionic gonadotropin (hCG) MAb have been used as investigation criteria in double-mouthed rolling bottle (DMRB). Compared with T-flask cell culture, both of the cell number and MAb production increased by approximately 42.5% when the medium was supplemented with 5% fetal calf serum (FCS) and D...
متن کاملA A Model of Fed-batch Culture of Hybridoma Cells
A mathematical model for fed-batch culture of hybridoma cells [24] has been employed for generating simulation data in this study. The model is a seventhorder nonlinear model where both glucose and glutamine concentrations are used to describe the specific growth rate, μ. The cell death rate, kd, is governed by lactate, ammonia and glutamine concentrations. The specific MAb production rate, qMA...
متن کاملEffect of serum starvation stress on the mouse spleen mononuclear cells mixed culture: Introducing a new immunomodulatory method
Introduction: Bone marrow and immune cell transplants are a common method of treating some blood diseases around the world. However, due to the malfunction of the immune system, its use is not always effective. In this study, we evaluated the potential of culture stress in serum-free medium in regulating the immune system. Materials and Methods: Spleen immune cells were isolated from Balb/C and...
متن کاملHybridoma cell culture optimization using nonlinear model predictive control
This work addresses the application of control systems to the optimization of a monoclonal antibodies (MAb) production chain. The attention is focused on the maximization of hybridoma fedbatch culture productivity. The proposed model presents kinetics showing strong nonlinearities through min-max functions expressing overflow metabolism. A nonlinear model predictive control (NMPC) algorithm, ch...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: KAGAKU KOGAKU RONBUNSHU
سال: 1988
ISSN: 0386-216X,1349-9203
DOI: 10.1252/kakoronbunshu.14.337